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Updated: June 21, 2025
The next experiment was to determine the volume of oxygen absorbed by a known quantity of yeast, the yeast living in contact with air, and under such conditions that the absorption of air was comparatively easy and abundant. The following are the data and results of an experiment of this kind. We employed 60 cc. A portion of the gas escaped and was collected in the jar.
Then his gaze fell upon the unhappy-looking old fellow on the hatch, who was holding his single shirt now in his hands, waiting for it to dry sufficiently for him to wear it again. As the rain fell in torrents every few minutes, this appeared an endless task, and the old man grew more sorrowful. "There ain't nothin' in this world fer me," said he, sadly, cc not even a bloomin' shirt.
As the tube, gg, dips into the water, it does not allow the gas to escape, and this permits of the revolution counter that the rod actuates being placed in an external case, CC'.
The solution of potato starch is less stable than the soluble starch. It is regarded as a "solid solution" of iodine in starch. Run out 40 cc. of the thiosulphate solution into a beaker, dilute with 150 cc. of water, add 1 cc. to 2 cc. of the soluble starch solution, and titrate with the iodine to the appearance of the blue of the iodo-starch.
"Yea," answered Beatrice; CC as sure as I have a thought or a soul." "Enough," said Benedick. "I am engaged; I will challenge him. I will kiss your hand, and so leave you. By this hand Claudio shall render me a dear account! As you hear from me, so think of me. Go, comfort your cousin."
The following is an experiment on this point: On August 15th, 1875, we sowed a trace of yeast in 150 cc. The solution was prepared in one of our double-necked flasks, with the necessary precautions to secure the absence of germs, and the yeast sown was itself perfectly pure.
To give extra security, drive ordinary pins, cut off to 5/8 inch, through the sides into fixed B, CC, and DD, and through EE into A. This is an easy enough business if pilot holes are made with a very fine awl or a tiny drill, and a small, light hammer is used.
We analyzed the gas immediately, and found that, allowing for the carbonic acid and hydrogen, it did not contain more than 14.2 per cent. of oxygen, which corresponds to an absorption of 6.6 cc., or of 3.3 cc. Lastly, we again established connection by an india- rubber tube between the flasks, after having seen by microscopical examination that the movements of the vibrios were very languid.
Taking into consideration the volume of this flask, this shows a minimum of 50 cc. The next experiment was to increase the proportion of oxygen to a still greater extent, by rendering the diffusion of gas a more easy matter than in a flask, the air in which is in a state of perfect quiescence.
Boil the solution for two or three minutes after the iron has dissolved to remove any volatile hydrocarbons. Meanwhile prepare the reductor for use as follows: Connect the vacuum bottle with the suction pump and pour into the funnel at the top warm, dilute sulphuric acid, prepared by adding 5 cc. of concentrated sulphuric acid to 100 cc. of distilled water.
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