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Updated: June 8, 2025
Pus was found in abundance by a peritoneal puncture and was sowed; so also was blood from a vein in the arm. The culture of pus yielded the long chains noted in the preceding observation and also the small pyogenic vibrio. The culture from the blood contained only the long chains. Third observation.
"It is very ingenious," said I, really appreciating the difficulty of being lively in this connection: it seemed even more wonderful than that a Vibrio should have an eye. "You are probably surprised to see no notices from the London press," said Vorticella. "I have one a very remarkable one. But I reserve it until the others have spoken, and then I shall introduce it to wind up.
This was what happened, but a new difficulty presented itself; all our cultures remained sterile. It occurred to us that the septic vibrio might be an obligatory anaerobe and that the sterility of our inoculated culture fluids might be due to the destruction of the septic vibrio by the atmospheric oxygen dissolved in the fluids.
It is certainly worthy of note that the uterus, peritoneum and intestines showed nothing special, but the liver was full of metastatic abscesses. At the exit of the hepatic vein from the liver there was pus, and its walls were ulcerated at this place. The pus from the liver abscesses was filled with the pyogenic vibrio.
Nor can it be supposed that there is a virus which loses its virulence at the moment that the adult vibrio dies; for such a substance should also lose its virulence when the vibrios, changed to germs, are exposed to the air. Since the virulence persists under these conditions it can only be due to the germ corpuscles the only thing present.
During this century of wonder a sufficiency of exactness was, however, introduced into the study of microscopic organisms to call for the use of names, and we find Muller using the names of Monas, Proteus, Vibrio, Bacillus, and Spirillum, names which still continue in use, although commonly with a different significance from that given them by Muller.
We may easily undeceive ourselves if we watch the movements of the vibrio, when we will readily recognize the bend, especially as it is brought into the vertical plane passing over the rest of the filament.
If we had found a wriggle, or a zigzag, or a shoot from one side to the other, in this last flask, what a scare there would have been, to be sure, in the schools of the prophets! Talk about your megatherium and your megalosaurus, what are these to the bacterium and the vibrio? These are the dreadful monsters of today.
Now, what bearing can the question of the animal or vegetable nature of the ferment, of the organized being, have upon the investigation of these two problems? In studying butyric fermentation, for example, we endeavoured to establish these two fundamental points; 1. The BUTYRIC FERMENT IS A VIBRIO. 2. M. Robin, however, would have no difficulty in determining the limits of the two kingdoms.
But when he had gone this question called from her a brief, ironical smile. She analyzed and criticised the physical and moral personality of the baron with perfect coolness, and at moments with a shade of contempt even. A vibrio!
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