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Updated: June 8, 2025
We have already said that we believe that they are nothing but the ordinary vibrios of putrefaction, reduced to a state of extreme tenuity by the special conditions of nutrition involved in the fermentable medium used; in a word, we think that the fermentation in question might be called putrefaction of tartrate of lime.
It is only needful to add a minute drop of fluid containing Bacteria, to water in which tartrate, phosphate, and sulphate of ammonia are dissolved; and, in a very short space of time, the clear fluid becomes milky by reason of their prodigious multiplication, which, of course, implies the manufacture of living Bacterium-stuff out of these merely saline matters.
We suppose that the substances employed, however pure, always contain some trace of iron, which becomes converted into the sulphide, the black colour of which would modify the originally white deposit of insoluble tartrate and phosphate. But what is the nature of these vibrios?
After the completion of fermentation there was not a trace of tartrate of lime remaining at the bottom of the vessel: it had disappeared gradually as it got broken up into the different products of fermentation, and its place was taken by some crystallized carbonate of lime the excess, namely, which had been unable to dissolve by the action of the carbonic acid.
The carbonic acid of the fermented liquid was then expelled by means of heat, and collected over mercury. Exactly half of the lime in the tartrate employed got used up in the soluble salts formed during fermentation; the other half was partly precipitated in the form of carbonate of lime, partly dissolved in the liquid by the carbonic acid.
Now, neutral tartrates, citrates, and acetates of the alkalis are found, in their passage through the system, to be changed into carbonates; and to convert a tartrate into a carbonate requires an additional quantity of oxygen, the abstraction of which must lessen the oxygen destined for assimilation with the blood, on the quantity of which the vigorous action of the human system partly depends.
A fungoid growth a unique one, of greenish-grey colour developed from spontaneous impregnation, and decolourized the liquid, which originally was of a yellowish- brown. Some large crystals, sparkling like diamonds, of neutral tartrate of lime, were precipitated, about a year afterwards, long after the death of the plant, we examined this liquid.
Tartaric acid is such a substance; and if a single spore of the commonest and most troublesome of moulds Penicillium be sown in a saucerful of water, in which tartrate of ammonia, with a small percentage of phosphates and sulphates is contained, and kept warm, whether in the dark or exposed to light, it will, in a short time, give rise to a thick crust of mould, which contains many million times the weight of the original spore, in protein compounds and cellulose.
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