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Updated: June 26, 2025


We do not know how far external circumstances influence the microscopic beings whose discovery is due to the unwearied patience of Hill, Baker, Joblot, Eichorn, Gleichen, Spallanzani, and especially of Muller, and last of all of M. Bory de Saint Vincent.

Cut off the legs, the tail, the jaws, separately or all together, and, as Spallanzani showed long ago, these parts not only grow again, but the redintegrated limb is formed on the same type as those which were lost. The new jaw, or leg, is a newt's, and never by any accident more like that of a frog.

Spallanzani joined issue with the English naturalist on both these pleas, and he showed that if, in the first place, the glass vessels in which the infusions were contained were hermetically sealed by fusing their necks, and if, in the second place, they were exposed to the temperature of boiling water for three-quarters of an hour, no animalcules ever made their appearance within them.

In the middle of the eighteenth century, it was revived, in a new form, by Needham and Buffon; but the experiments of Spallanzani enforced the conclusions of Redi, and compelled the advocates of the occurrence of spontaneous generation to seek evidence for their hypothesis only among the parasites and the lowest and minutest organisms.

A few men of more strictly scientific natures paid some attention to these little organisms. Among them we should perhaps mention Von Gleichen, Muller, Spallanzani, and Needham. Each of these, as well as others, made some contributions to our knowledge of microscopical life, and among other organisms studied those which we now call bacteria.

Accordingly he made numerous experiments, always with the same results that after a few days the water was found to swarm with the microscopic creatures. The thing seemed proven beyond question providing, of course, that there had been no slips in the experiments. But Abbe Spallanzani thought that he detected such slips in Needham's experiment.

Once more, an Italian, the Abbé Spallanzani, a worthy successor and representative of Redi in his acuteness, his ingenuity, and his learning, subjected the experiments and the conclusions of Needham to a searching criticism. It might be true that Needham's experiments yielded results such as he had described, but did they bear out his arguments?

Cut off the legs, the tail, the jaws, separately or all together, and as Spallanzani showed long ago, these parts not only grow again, but the new limb is formed on the same type as those which were lost. The new jaw, or leg, is a newt's, and never by any accident more like that of a frog's."

Cut off the legs, the tail, the jaws, separately or all together, and, as Spallanzani showed long ago, these parts not only grow again, but the redintegrated limb is formed on the same type as those which were lost. The new jaw, or leg, is a newt's, and never by any accident more like that of a frog.

The possibility of such slips might come in several ways: the contents of the jar might not have been boiled for a sufficient length of time to kill all the germs, or the air might not have been excluded completely by the sealing process. To cover both these contingencies, Spallanzani first hermetically sealed the glass vessels and then boiled them for three-quarters of an hour.

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