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This indicator cannot, however, be successfully used with weak bases, even ammonium hydroxide; for, since it is weak acid, the salts which it forms with weak alkalies are easily hydrolyzed, and as a consequence of this hydrolysis the change of color is not sharp. This indicator is affected by even so weak an acid as carbonic acid, which must be removed by boiling the solution before titration.
The history of science was making fast, and I was interested in a thousand subjects. Why, there was my theory of the hydrolysis of casein by trypsin, which Professor Walters had been carrying out in his laboratory. Also, Professor Schleimer had similarly been collaborating with me in the detection of phytosterol in mixtures of animal and vegetable fats.
I can see nothing wrong with the blood; it has not coagulated as I expected, nor is there any pronounced hydrolysis as yet. But I'm afraid I'll have to write out the death warrants for all these men and women. One of the people on that ship was coming to see me. That's how I happened to be on the field. For her, at least, it may be better so. The poor woman was suffering from an incurable cancer."
This process repeats itself with great rapidity until, if sufficient acid is present, the solid passes completely into solution. Exactly the same sort of process takes place if calcium oxalate, for example, is dissolved in hydrochloric acid. The term !hydrolysis! is applied to the changes which result from the reaction of these ions. The solution exhibits an acid reaction.
It is the indicator most generally employed for the titration of organic acids. In general, it may be stated that when a strong acid, such as hydrochloric, sulphuric or nitric acid, is titrated against a strong base, such as sodium hydroxide, potassium hydroxide, or barium hydroxide, any of these indicators may be used, since very little hydrolysis ensues.
That methyl orange should be most sensitive toward alkalies is evident from the following considerations: Methyl orange is a weak base and, therefore, but little dissociated. Comparison of this equation with that on page 30 will make it plain that hydrolysis is just the reverse of neutralization and must, accordingly, interfere with it.
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