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The dry mixture is then transferred to a large glass tube, having a small hole in the bottom plugged with glass wool to act as a filter, and light petroleum spirit which boils at about 150 deg. to 180 deg. Fahr. is poured over it, till all the neutral or unsaponifiable oil is dissolved out.
All animal and vegetable oils yield a small quantity about one per cent. of unsaponifiable fatty matter, which must be deducted from the result obtained. Sperm oil, however, was found to be an exception, because from its peculiar chemical constitution it yields nearly half its weight of a greasy substance to the ether or to the petroleum spirit.
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