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He never married again, but a beautiful bourgeoise, Laura Eustochia Dianti, became his mistress. She bore him two sons, Alfonso and Alfonsino. The duke died October 31, 1534, at the age of fifty-eight; his brothers, Cardinal Ippolito and Don Sigismondo, having passed away before him, the former in 1520 and the latter in 1524. By Lucretia Borgia he had five children.
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