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The tetanus bacillus, which is a perfect anaërobe, is widely distributed in nature and can be isolated from garden earth, dung-heaps, and stable refuse. The spores, which are the active agents in producing tetanus, are highly resistant to chemical agents, retain their vitality in a dry condition, and even survive boiling for five minutes.
The anaërobe most constantly present is the bacillus ærogenes capsulatus, usually in association with other anaërobes, and sometimes with pyogenic diplo- and streptococci. According to the mode of action of the associated organisms and the combined effects of their toxins on the tissues, the gangrenous process presents different pathological and clinical features.
"Yesterday I found that anaerobe cultures were being received by some one in the Belleclaire, and " "They were stolen from me. Some one must have got into my office, where I was studying them." Doctor Gavira had pressed forward earnestly, but Craig did not pause again. "Who were these agents sent over to wage this secret war at any cost?" he repeated.
This was what happened, but a new difficulty presented itself; all our cultures remained sterile. It occurred to us that the septic vibrio might be an obligatory anaerobe and that the sterility of our inoculated culture fluids might be due to the destruction of the septic vibrio by the atmospheric oxygen dissolved in the fluids.
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