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The following is an experiment on this point: On August 15th, 1875, we sowed a trace of yeast in 150 cc. The solution was prepared in one of our double-necked flasks, with the necessary precautions to secure the absence of germs, and the yeast sown was itself perfectly pure.
Clearly then, the purity of the yeast at the moment of impregnation, and the purity of the liquid in the funnel, are conditions indispensable to success. Thus fitted, the funnel can answer the same purposes as our double-necked flasks.
It is possible to produce a ready entrance and escape of pure air in the case of the double-necked flasks which we have so often employed in the course of this work, without having recourse to the continuous passage of a current of air. In this manner we may increase or prolong the fructification of fungoid growths, or the life of the aerobian ferments in our flasks.
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